Journal: Pharmaceutics
Article Title: Elovanoids Counteract Inflammatory Signaling, Autophagy, Endoplasmic Reticulum Stress, and Senescence Gene Programming in Human Nasal Epithelial Cells Exposed to Allergens
doi: 10.3390/pharmaceutics14010113
Figure Lengend Snippet: ELVN-34 reduce the expression of pro-inflammatory cytokines, chemokines and cell adhesion molecule in HNEpC challenged with LPS or poly(I:C). HNEpC challenged with LPS or poly(I:C) (30 μg/mL) display enhanced production of pro-inflammatory cytokines and chemokines IL-6, IL-1β, IL-8/CXCL8, CCL2/MCP-1, CXCL1/KC/GRO, VEGF, and cell adhesion molecule ICAM1(CD54) compared to non-treated cells. This increase in the production of pro-inflammatory molecules is abrogated by the addition of ELVN-34 (500 nM) 30 min post-challenge. LPS and poly(I:C) induce a reduction of anti-inflammatory IL-10 expression in HNEpC that is restored to normal levels after treatment with ELVN-34 (500 nM). The results showed the averages of three independent experiments. (**** p < 0.0001, NS—not significant).
Article Snippet: In addition, the expression of the following cytokines was analyzed in the supernatant of HNEpC exposed to different allergic inductors: IL-6 (Catalog# 6802, Chondrex) range of detection: (9–600 pg/mL), IL-1β (Catalog# 6805, Chondrex) range of detection: (4–250 pg/mL) IL-8/CXCL8 (Quantikine ELISA Kit, Catalog# D8000C, R&D Systems) range of detection: (31–2000 pg/mL), vascular endothelial growth factor (VEGF) (Catalog# 6810, Chondrex) range of detection: (31–2000 pg/mL), ICAM1(CD54) (ELISA Kit, Catalog# ab100640, Abcam) range of detection: (16–1000 pg/mL), CXCL1/KC/GRO (Catalog# 6825, Chondrex) range of detection: CCL2/MCP-1 (Catalog# 6821, Chondrex) range of detection: (16–1000 pg/mL), and IL-10 (Catalog# 6806, Chondrex) range of detection: (8–500 pg/mL).
Techniques: Expressing